The slides were analyzed using a Carl Zeiss Axioplan 2 microscope with a CCD camera (CV M300, JAI, Kushima, Miyazaki, Japan), CHROMA filter sets, and ISIS4 image-processing package (MetaSystems GmbH, Altlussheim, Germany).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.