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In order to identify 3′ and 5′ ends of AlstR mRNA, Rapid Amplification of cDNA Ends (RACE) was performed. The PCR reactions were performed by 5′/3′ RACE Kit 2nd Generation (Roche, Basel, Switzerland) according to the manufacturer’s instructions. For 5′ RACE specific primers were SP1 5′-AAGCGGAGCACCACGTAGAT-3′ and SP2 5′-GACGAGGCCGTTGCCGAGCAG-3′. For 3′ RACE specific primers were SP4 5′-CTGCACATGGAGAACAGCGTG-3′ and SP5 5′-TTCCCGCGGCGCCACCCCGG-3′.
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