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SS Shwetha Srinivasan
RR Raju Regmi
XL Xingcheng Lin
CD Courtney A. Dreyer
XC Xuyan Chen
SQ Steven D. Quinn
WH Wei He
MC Matthew A. Coleman
KI Kermit L. Carraway, III
BZ Bin Zhang
GS Gabriela S. Schlau-Cohen
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Statistical analysis was performed using MATLAB. One-way analysis of variance (ANOVA) was performed on different pairs of single-molecule FRET data and statistical significance was set at P ≤ 0.001. The P-values, degrees of freedom, F-statistics is reported in Supplementary Table 10. The median, minimum, maximum, whiskers, and quartiles for the single-molecule FRET distributions is provided in Supplementary Tables 11, 12. The number of data points in the smFRET lifetime distributions is reported in Supplementary Table 13. Two-tailed, nonparametric, paired t-test was performed on different pairs of western blot phosphorylation experiments and the P-values are reported in Supplementary Tables 7, 14. The average, standard deviation, and standard error of mean for the western blot distributions is reported in Supplementary Tables 6, 15.

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