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Immunofluorescence

JJ Jennifer Jung
AN Arnab Nayak
VS Véronique Schaeffer
TS Tatjana Starzetz
AK Achim K Kirsch
SM Stefan Müller
ID Ivan Dikic
MM Michel Mittelbronn
CB Christian Behrends
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After fixation with 4% paraformaldehyde, cells were permeabilized with 0.5% Triton-X 100 in PBS (10 min), followed by blocking with 1% BSA in PBS for 1 hr. Primary and secondary antibodies as well as nuclear and cytoplasmic staining reagents (AlexaFluor-coupled antibodies (Life Technologies); DRAQ5 (Cell Signaling); HSC CellMask Deep red stain (Life Technologies)) were incubated in 0.1% BSA in PBS for 1 hr with three washes of PBS in between. For double stainings, antibodies were incubated sequentially.

Copyright and License information:  ©2017, Jung et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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