Isoproterenol pump implantation and Mdivi-1 treatments

For all studies, 8- to 12-week-old male C57BL/6 or MITOL KO mice were randomly assigned to receive i.p. injections of either isoproterenol (60 mg/kg, #19703-04, nacalai tesque) or saline for 7 days using mini-osmotic pumps (model: alzet-20; Durect Corporation, CA. USA). Pumps were dorsally implanted in the mice for 7 days. Mice received once daily i.p. injections (50 mg/kg) with mdivi-1 beginning on the day of the first isoproterenol treatment and continued until mice were collected 7 days. At the end of the treatment period, pumps were surgically removed.