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2.2. 16S rRNA Sequencing and PICRUSt Pathway Analysis
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Fecal samples from healthy subjects (n = 8) and PE patients (n = 6) were collected to detect changes in microbial diversity. Illumina NovaSeq PE250 was applied for 16S amplicon sequencing to obtain raw data. Sequence data analysis mainly used Qiime 2 (Qiime2-2020.2) and R software (4.0.2). In addition, KEGG pathway analysis was performed by PICRUSt.
Copyright and License information: ©2022 Rong Tang et al.
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