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2.6. Acridine Orange/Ethidium Bromide (AO/EB) Staining
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A glass cover slip was preplaced in a 6-well plate to make cell slide. The cells were fixed with 95% ethanol at room temperature for 15 min. 100 mg/L of AO (Sigma, St.Louis, MO) dissolved in PBS and 100 mg/L of EB (Sigma, St.Louis, MO) dissolved in PBS were mixed, each with 5 μL, and then immediately added to the cells. After 30 s, it was observed using a confocal laser microscope (LSCM880NLO, Zeiss, Germany).
Copyright and License information: ©2022 Shu-wen Sun et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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