2.1. The Establishment of the Trimeresurus stejnegeri Poisoning Rabbit Model

The Japanese rabbits were purchased from the Charles River Laboratories (Beijing, China) and the Trimeresurus stejnegeri venom was purchased from Fuzhou Wanlong Biotechnology Co., LTD (Fujian, China). After adapting for 7 days, rabbits were intramuscularly injected with 1.5 mg/kg, 2 mg/kg, 2.5 mg/kg, 11.5 mg/kg, 20 mg/kg, and 40 mg/kg Trimeresurus stejnegeri venom, respectively. The initial toxic dose was found to be 20 mg/kg, which was used as the dosage to establish the Trimeresurus stejnegeri poisoning rabbit model. Animals in the model group (n = 6) were injected with 20 mg/kg Trimeresurus stejnegeri venom and those in the control group (n = 6) were injected with the equal volume of normal saline. The blood was collected from the heart 4 hours later and the serum was separated, which was used for proteomics analysis and Western blot identification. This study was approved by the Ethics Committee of People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine (FTCM2022-011).