2.5. Creation of Wound Infection in Weightless Rats

The weightless rats were depilated on the back after anesthesia with urethane (30%, 0.7 ml/100 g). Two excised wounds were created by cutting a full-thickness skin with a diameter of 1.5 cm from a predetermined area on both sides of the dorsal region of the midline to the depth of loose subcutaneous tissue [23]. The E. coli suspension (ATCC 25922, 10⁸ cfu/ml) was dropped via pipette to induce skin infection [24, 25]. 1 ml of the suspension was used for each wound except the uninfected group. The wound was covered with medical transparent film, wrapped, and fixed with sterile antilicking gauze.