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PacBio Sequel II Data Generation

MS Mudagandur S. Shekhar
VK Vinaya Kumar Katneni
AJ Ashok Kumar Jangam
KK Karthic Krishnan
SP Sudheesh K. Prabhudas
JA Jesudhas Raymond Jani Angel
KS Krishna Sukumaran
MK Muniyandi Kailasam
JJ Joykrushna Jena
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Genomic DNA was extracted from the muscle tissue of M. cephalus using the blood and cell culture DNA midi kit (Qiagen). DNA quantification was carried out using a Qubit 4.0 fluorometer (Thermofisher Scientific). Library preparation was performed with the SMRTbell® Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, California, United States), and size selection was carried out using BluePippin™ (Sage Science, Beverly, Massachusetts, United States). The library was sequenced on the PacBio Sequel II platform (Pacific Biosciences) to generate the sequence data. About 257.7 Gb of sequence data in 15,028,480 subreads was generated with a subread N50 of 28,748 bp (Supplementary Table S2).

Copyright and License information:  ©2022 Shekhar, Katneni, Jangam, Krishnan, Prabhudas, Jani Angel, Sukumaran, Kailasam and Jena.
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