4.1. Bothrops Moojeni Venom, Cell Lines and Ethics Committee Guidelines

B. moojeni crude venom was collected from four adult specimens from the Serpentarium at the Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil, by direct pressure on the snake venom glands. The cell lines used were as follows: the healthy human fibroblast cell line (MRC-5), the human colorectal cancer cell line (Caco-2), human rhabdomyosarcoma cells (RD) and mucoepidermoid carcinoma of the lung (NCI- H292), which were obtained from the Adolf Lutz Institute, and microglia cells (BV-2) were acquired from the Cell Bank of Rio de Janeiro. Mice (Mus musculus) erythrocytes were also used for assays, approved by the animal ethics committee from the Universidade Católica Dom Bosco under registration number 014/2018.