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Hemin (Ferriprotoporphyrin IX chloride, Sigma-Aldrich) was dissolved in 0.1 M NaOH, neutralized (to pH 7.2) with 1 M HCl and adjusted to concentration of 7.7 mM with distilled water. Aliquots were protected from light and stored at −80 °C until used. Hemin was then diluted in sterile saline (NaCl 0.9%) to appropriate concentration and filtered.
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