2.8. Statistical Analysis

Statistical analysis was performed using GraphPad Prism^® software (version 6). One-way analysis of variance (ANOVA) followed by a Tukey’s post hoc test was used to identify differences between males and females in weekly average body weight during the study. The same combination of analyses was also conducted to determine any significant differences in behavioral and molecular data between groups. A value of p < 0.05 was considered significant. Unpaired t-tests were conducted to compare brain inflammatory markers between the E-cig Nicotine group and the Air Control group, and also to compare behavioral parameters between female and male mice in the E-cig Nicotine group. T-test was used to compare between number of enteries into the same object zone and number of enteries into the novel object zone within each group.