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Relative Pixel Quantification

KA Kwaku Dad Abu-Bonsrah
DZ Dongcheng Zhang
DN Donald F. Newgreen
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Standard confocal images were selected and analysed using the Zeiss Image Analyser. Regions of interest were selected with the Free-Hand tool and choosing the DAPI channel, the cells in a field were counted and relative pixel quantification was calculated following a previous publication64. Relative fluorescence intensity was calculated by normalising the mCherry and DGCR8 expression to DAPI intensity.

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