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HCMEC/D3 cells (FuHeng Biology, FH1110, Shanghai, China) were cultured in endothelial cell medium (ECM,ScienCell, Carlsbad, United States) and added fetal bovine serum (FBS, Gibco, Australia) into ECM to making its concentration to 10%, and then incubated the cells in humidified atmosphere at 37°C with 5% CO2. All experiments with hCMEC/D3 cells were used below 15 passages.
Copyright and License information: ©2022 Jiang, Li, Cai, Liu, Chen, Xu, Deng, Sun, Zhou, Huang, Wu and Cheng.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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