2.10. qRT-PCR

Total RNA of kidney tissues was extracted using TRIzol reagent (Invitrogen, CA, USA) and were reversely transcribed to cDNA using the PrimeScript One-Step RT-PCR Kit (Takara, Japan). qRT-PCR was performed using the SYBR Green qPCR master Mix kit and the Agilent Stratagene Mx3000P Real-Time PCR instrument (DBI Bioscience, China). Reaction program was 95°C for 3 min, and 40 cycles of 95°C for 12 s and 62°C for 40 s. The primers used in this work were listed in Table S1. The relative mRNA expression of target genes was calculated using the 2^−∆∆Ct method by normalizing to GAPDH.