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The cells were continuously superfused with a HEPES-buffered Tyrode solution containing (in mmol/L): NaCl 145, KCl 4, MgCl2 1, CaCl2 1, HEPES 5, glucose 5, pH adjusted to 7.4 with NaOH. Patch pipettes were filled with the following solution (in mmol/L): KCl 100, Kgluconate 45, MgCl2 1, EGTA 5, HEPES 10, pH adjusted to 7.2 with KOH. The membrane-permeable oxidizing agent tert-butylhydroperoxide (tbHO2) and dithiothreitol (DTT) were obtained from Sigma. Incubations with tbHO2 and DTT were realized at room temperature.
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