Rotarod test

The procedures of the rotarod have been described previously (Goldberg et al., 2003). Briefly, four mice at a time were placed on an Economex accelerating rotarod (Columbus Instruments) equipped with individual timers for each mouse. Mice were initially trained to stay on the rod at a constant rotation speed of 5 rpm. After a 2 min rest, mice that had fallen were repeatedly placed back on the rotarod until they were able to stay on the rotating rod for at least 2 min. Following training, mice were subsequently tested by placing them on the rod at a rotation speed of 5 rpm, and as the rod accelerated by 0.2 rpm/s, the latency to fall was measured. Mice were tested for a total of three trials. Between trials, mice were placed in the home cage for 2 min to rest.