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Luciferase reporter assay

SK Samrat T. Kundu
BR B. Leticia Rodriguez
LG Laura A. Gibson
AW Amanda N. Warner
MP Mabel G. Perez
RB Rakhee Bajaj
JF Jared J. Fradette
CC Caleb A. Class
LS Luisa M. Solis
FA Frank R. Rojas Alvarez
IW Ignacio I. Wistuba
LD Lixia Diao
FC Fengju Chen
MS Mohit Sachdeva
JW Jing Wang
DK David G. Kirsch
CC Chad J. Creighton
DG Don L. Gibbons
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For reporter assays, respective cells were cotransfected with 500 ng of the reporter construct (GL3) with 10 ng of control (RL) constructs for 16 h with Lipofectamine LTX (Thermo Fisher Scientific) and then assayed for luciferase activity after 48 h of doxycycline induction using the dual-luciferase reporter assay system (Promega E1980).

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