Generation of F4 and F5 progeny from P. sojae cross ACR10 X P7076

Selected F₃ progeny with the genotype Avr3a^P7076/Avr3a^P7076 were available from the cross ACR10 X P7076. These cultures were self-fertilized to produce F₄ and F₅ progeny. The original ACR10 X P7076 cross was previously performed and the progeny were maintained in the laboratory [30]. A total of four F₃ individuals (F₃-40, F₃-60, F₃-83, and F₃-87) were revived from cryo-storage, and self-fertilized to generate F₄ progeny; similarly, F₅ progeny were developed from the F₄ progeny.