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hESC-ECs were incubated with Dil fluorescent dye-labeled acetylated low-density lipoprotein (Dil-Ac-LDL) (Biomedical Technologies, USA) at 10 μg/mL in ECM (ScienCell, USA) for 4 h at 37 °C. After washing three times with PBS, cells were subjected to immunofluorescence staining, and images were acquired with a Zeiss LSM880 confocal laser scanning microscope (Carl Zeiss, Germany).
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