Describe sample preparation and provide justification for the choice of the suspending medium (vehicle) which should be compatible with the assay used. Due to solubility issues, NM are often present as a dispersion of particles. Information should be provided on handling of the NM, such as sonication of the suspension. Suspensions of the NM test article should be prepared just before use in the assay. Description of NM storage and the potential for change in properties during storage should be considered.
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