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Monolayer cultures of DF-1 cells were seeded at 50–60% confluence in 12-well plates and infected with rLS1 or rLS1- eGFP viruses at a (MOI) of 0.05. Cells were cultured with DMEM containing 1% FBS and 5% AF with 5% CO2 at 37 °C. Supernatants were collected 12, 24, 36, 48, 60 and 72 h post-infection (h.p.i.). Collected supernatants were quantified in DF-1 cells by plaque assay as described above.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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