miRNA transfection

Cells were cultivated in a 6-well cell culture plate (10⁶ cells/well) and were then transfected with 1ng lncRNA-SNHG7 plasmid (GenePharma Co., Ltd., Shanghai, China), 1ng control plasmid, 100 nM miR-449a mimics (Guangzhou Ribobio Co., Ltd, Guangzhou, China), 100 nM mimics control (Guangzhou Ribobio Co., Ltd), 50 nM miR-449a inhibitor (Guangzhou Ribobio Co., Ltd), 50 nM control inhibitor (Guangzhou Ribobio Co., Ltd), 0.2 µM control-small interfering RNA (control-siRNA; Santa Cruz Biotechnology, Dallas, TX, USA), or 0.2 µM si-TNIP2 (Santa Cruz Biotechnology, Dallas, TX, USA) using Lipofectamine® 2000 (Life Technologies Corporation, Savant, MA, USA), according to the manufacturer’s instructions.