JC-1 Staining

Mitochondrial membrane potential assay kit with JC-1 (C2006, Beyotime, China) was used to observe the entire cell morphology and compare the changes of mitochondrial membrane potential between different groups. 50 μL JC-1 (200 ×) was diluted with 8 mL ultrapure water, and then added 2 mL JC-1 staining buffer (5 ×), the mixture was JC-1 staining working solution. 1 mL JC-1 staining working solution was added to the culture plate and incubated in the cell culture box at 37°C for 20 min. After incubation, they were washed twice with JC-1 dyeing buffer (1 ×) and photographed for observation.