Determination of adhesion molecule expression on resting and stimulated neutrophils

CL Chloé Loyer
AL Arnaud Lapostolle
TU Tomas Urbina
AE Alexandre Elabbadi
JL Jean-Rémi Lavillegrand
TC Thomas Chaigneau
CS Coraly Simoes
JD Julien Dessajan
CD Cyrielle Desnos
MM Mélanie Morin-Brureau
YC Yannick Chantran
PA Pierre Aucouturier
BG Bertrand Guidet
GV Guillaume Voiriot
HA Hafid Ait-Oufella
CE Carole Elbim
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Heparin whole-blood samples (500 μL) were either kept on ice or incubated with PBS or 10−6 M bacterial peptide formyl-methionyl-leucyl-phenyl-alanine (fMLP) (Sigma Chemical Co., St Louis, MO) for 5 min. Samples were stained with PE-anti-human CD11b (clone 2LPM19c, Dakopatts, Glostrup, Denmark) and APC-anti-human CD62L (clone DREG-56, BD Biosciences) as previously reported [22]. Samples were then analyzed by means of flow cytometry, as described in the Additional file 1: Methods.

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