2.1. Cell Culture

FH Florian D. Hastert
SH Sascha Hein
CR Christine von Rhein
NB Nuka Ivalu Benz
YH Younes Husria
DO Doris Oberle
TM Thorsten J. Maier
EH Eberhard Hildt
BS Barbara S. Schnierle
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HEK293T (ATCC CRL-3216) and HEK293T-hACE2 cells [11] were grown in Dulbecco’s Modified Eagle Medium (DMEM; Sigma, Taufkirchen, Germany) supplemented with 10% foetal bovine serum (Sigma, Taufkirchen, Germany), 5% l-glutamine (200 mM; Lonza, Verviers, Belgium), and 1% penicillin/streptavidin (Fisher Scientific, Schwerte, Germany) at 37 °C under 5% CO2. The medium for HEK293T-hACE2 cells was furthermore supplemented with 50 µg/mL Zeocin™ (Fisher Scientific, Schwerte, Germany).

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