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ALP and TGFβ1 determination

GL Giovanni Longo
CI Caterina Alexandra Ioannidu
Ad Anna Scotto d’Abusco
FS Fabiana Superti
CM Carlo Misiano
RZ Robertino Zanoni
LP Laura Politi
LM Luca Mazzola
FI Francesca Iosi
FM Francesco Mura
RS Roberto Scandurra
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After culturing the cells on Ti and on TiC, for the time required for the different experimental procedures, we lysed the cells and collected the cell supernatants. We measured the concentration (pg/ml) of Transforming Growth Factor-β1 (TGF-β1) in the cell supernatants by using TGF-β1 EIA kit (ADI-900-155).

The Alkaline Phosphatase (ALP) activity (U/ml) was measured in cell lysates using ALP assay colorimetric (kit ab83369). The experiments were conducted according to manufacturers’ instructions

Copyright and License information:  ©2016 Longo et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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