Determination of Malondialdehyde (MDA) content, and Membrane Stability Index (MSI)

Malondialdehyde content was determined by grinding 0.1 g frozen leaf in 25 mL phosphate buffer solution (50 mM) having 1% polyethylene pyrrole at 4°C followed by centrifugation at 10,000 × g for 15 minutes. The supernatant was subjected to heating at 100°C for 15 minutes and quickly cooled in an ice bath. Absorbance was taken in a spectrophotometer at 450, 532, 600 nm [46]. Fresh samples were used to calculate the membrane stability index by following the method of Sairam [47].