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Target-encoding plasmids were transfected into HEK 293T cells cultivated within the 24-well plates using Lipofectamine 3000. Twenty-four hours later, cells were gathered with the purpose of measuring their luciferase activities by adopting a Dual-Glo luciferase assay system (DL101; Vazyme Biotech Co., Ltd.), with Renilla luciferase being a reference.
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