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Analysis of Mass Spectrometric Data

TX Tao Xu
JC Jingyao Chen
KY Kai Yang
WQ Weicang Qiao
JZ Junying Zhao
LC Lijun Chen
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The raw data files of the obtained samples were compared with the database using Thermo Proteome Discoverer 1.4 software. SEQUEST HT was used as the search software of the comparison database, and the database was derived from UniProt (Homo sapiens and Bos taurus were used for the human and cattle, respectively). The specific parameters were set as follows: enzyme of trypsin (full), maximum missed cleavage site of 2, parent ion mass deviation of 10 ppm, and daughter ion mass deviation of 0.02Da. Dynamic modification was set as oxidation/M +15.995 Da and deamidation/N, Q +0.984Da, and fixed modification was set as carbamidomethyl/C +57.021Da.

Copyright and License information:  ©2022 Xu, Chen, Yang, Qiao, Zhao and Chen.
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