The napsin A levels in both ELF (ELF-napsin A) and serum (serum-napsin A) were measured by enzyme-linked immunosorbent assay with the Human Napsin A Assay Kit (Immuno-Biological Laboratories Co., Gunma, Japan). The CEA levels in ELF (ELF-CEA) were determined by chemiluminescent enzyme immune assay with Lumipulse Presto CEA (Fujirebio Inc., Saitama, Japan), and those in serum (serum-CEA) were quantified by electro-chemiluminescence immune assay with Cobas 800 (Roche Diagnostics K.K., Tokyo, Japan). Both ELF-napsin A and ELF-CEA were expressed per unit volume after correction for the dilution factor.
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