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Histopathological examination
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Histopathological examination was performed for the assessment of cardiac injuries. Tissue samples from each group were fixed in a fixative containing absolute alcohol (85 ml), glacial acetic acid (5 ml) and 40% formaldehyde (10 ml). After dehydration, tissue samples were fixed in paraffin to prepare blocks for microtomy. Tissues were sectioned 4–5 μm with microtome and stained with hemotoxilin-eosin (H&E) and were examined under light-microscope-(DIALUX 20 EB) at 40X.
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