Bacterial swimming motility assay

WL Wei Liu
XT Xiu-Qi Tian
JW Jin-Wei Wei
LD Li-Li Ding
WQ Wei Qian
ZL Zhong Liu
FW Fang-Fang Wang
request Request a Protocol
ask Ask a question
Favorite

The assayed strains were cultured overnight at 28 °C in rich NYG medium with the addition of different concentrations of IPTG or arabinose, washed twice with 10 mM MgCl2, and diluted to an OD600 nm = 0.4. Three microliters of the diluted cultures were then used to inoculate rich NYG medium plates containing 0.15% agar. The diameters of the swimming zones were measured after incubation of the plates at 28 °C for 24 h.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A