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An expression vector driven by a cytomegalovirus promoter was constructed for transfection of human S100A6‐N‐emGFP, in which the human S100A6 sequence was fused with emGFP at the N‐terminus to create a fusion protein. GUS‐N‐eGFP was used as the empty plasmid for the control group. The plasmid vector was acquired through SIDNET (Toronto, ON, Canada) and was sequence‐verified prior to use in vivo.
Copyright and License information: ©2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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