4.7. Blood Agar Hemolysis

Bacteria were streaked from a glycerol stock onto sheep blood agar plates and incubated overnight at 37 °C. The next day, single colonies were spotted onto tryptic soy agar (TSA) plates containing 3% of sheep, rabbit (both from Quad Five, Ryegate, MT, USA), or human blood (Department of Transfusion Medicine, NIH, Bethesda, MD, USA). Plates were incubated overnight at 37 °C and photographed the next day.