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Antimicrobial activity was investigated against the chosen microorganisms using the disk diffusion method as described previously.36 The McFarland 3 turbidity standard was used as a reference to inoculate fresh LB Agar plates with 9×108 colony forming units (CFUs) of each microorganism in broth, which were later evenly spread on the medium surface. A 7 mm diameter disk was obtained from each membrane sample and placed onto the inoculated plates and incubated at 37°C for 18 hours.
Copyright and License information: ©2017 Hassiba et al. This work is published and licensed by Dove Medical Press Limited
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