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DharmaFECT DUO reagent, non-targeting siRNA, or adiponectin siRNA (all from GE Dharmacon, Lafayette, CO) were pre-incubated with OptiMEM media (Life technologies Carlsbad, CA) according to the manufacturer’s instructions. Mature adipocytes were trypsinized. 1.16 X105 cells/cm2 were re-plated in 12-well plates and transfected by adding transfection reagents. After 24 h, media was changed to DMEM with 10% FBS. FFAs and UT treatments were added 48 hours after transfection.
Copyright and License information: ©2016 Obanda et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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