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Histochemical staining assay
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3,3’-diaminobenzidine (DAB) staining and trypan blue staining were performed as previously described [53, 54] on 5-leaf old seedlings cultivated in a phytotron for 3 days under 16 h of light / 8 h of dark at 4°C. The leaves from individual lines were used for DAB staining and trypan blue staining. Three biological replicates were performed.
Copyright and License information: ©2016 Qiu et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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