Plasmid constructs

Vectors pTRV1 (Liu et al., 2002) and pTRV2-LIC (Dong et al., 2007) were described previously. pTRV2-NbATG5 and pTRV2-NbATG7 were described (Wang et al., 2013). VIGS construct of NbGAPCs was described (Han et al., 2015). pTRV2-NbJoka2 was generated by cloning NbJoka2 cDNA fragment into pTRV2-LIC. Approximately 200 bp of mGFP5-ER were cloned into pTRV2 by specific primers.

The full-length infectious clones of CLCuMuV ({"type":"entrez-nucleotide","attrs":{"text":"GQ924756.1","term_id":"300509541","term_text":"GQ924756.1"}}GQ924756.1) and CLCuMuB ({"type":"entrez-nucleotide","attrs":{"text":"GQ906588.1","term_id":"300509539","term_text":"GQ906588.1"}}GQ906588.1) were described (Jia et al., 2016). The full-length infectious clones of TYLCCNV and TYLCV were described (Tao and Zhou, 2004; Zhang et al., 2009).

βC1 and its mutant βC1^V32A were cloned into pGEX4T-1 vector to express GST-tagged fusion proteins in Escherichia coli. Full-length cDNA of NbATG8f was cloned into pET28a to express NbATG8f-6×His in E. coli.

CFP-NbATG8f, GFP-NbATG8f, GFP-NbATG8c, GFP-NbATG8d, GFP-NbATG8i, cYFP-NbATG8f, nYFP-βC1, GFP-βC1, YFP-βC1, HA-βC1, HA-nLUC, nYFP-βC1^V32A, GFP-βC1^V32A, YFP-βC1^V32A, HA-βC1^V32A, and HA-nLUC were obtained respectively by overlapping PCR, and then cloned between the duplicated CaMV 35S promoter and the NOS terminator of pJG045, a pCAMBIA1300-based T-DNA vector (Du et al., 2013).