4.1. Mice

HPSE-1-deficient C57BL/6J mice were kindly provided by Dr. I Vlodavsky and Dr. J Li [18]. C57BL/6J OT-I, OT-II, and RIP-OVA^hi mice [9,29] were obtained from the Walter and Eliza Hall Institute (WEHI), Australia. As the OT-II TCR is linked to the Y chromosome, only male donor and recipient mice were used. C57BL/6J mice normally express the CD45.2 allele of CD45 but a CD45.1 C57BL/6J strain has been developed that provides CD45.1 as a convenient marker for tracking injected leukocytes, with the Australian Phenomics Facility (ANU) breeding CD45.1 bearing strains of the ones listed above (and as indicated in the Figures). All mice were maintained in the specific-pathogen-free Australian Phenomics Facility (ANU). All animal procedures were approved by the Australian National University Animal Experimentation Ethics Committee (AEEC).