2.6. 3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assay

Cells were cultured in 96-well plates at 2 × 10³ cells/well and transfected with siRNA containing lentivirus for 1–5 days. At the end of each incubation period, 20 µL of MTT solution (5 mg/mL) were added to each well and incubated at 37 °C for 4 h. The formazan crystals were dissolved in 200 µL of acidic isopropanol (10% SDS, 5% isopropanol. and 0.01 M HCl). Finally, the optical density was measured using a microplate reader at 595 nm.