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Line scans were performed by drawing a three pixel width line along the actin filament and recording the fluorescence intensity along the line using ‘Plot Profile’ in FIJI (Schindelin et al., 2012; Schneider et al., 2012). The same region of interest was then applied to the ABP channel of interest. If necessary, the region of interest was adjusted to account for filament movement during channel switching.
Copyright and License information: ©2017, Christensen et al
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