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To visualize the wound healing process, epithelial cells from the EM and the ECX were plated in 96-well plates and scratched as described above. Immediately after scratch, plates were inserted in the IncuCyte Zoom system (Essen Bioscience, Ann Arbor, MI) to acquire images. Serial images were taken every two hours for EM epithelial cells and every 15 min for ECX epithelial cells, for a 24–48 h period with a 10X objective. Images were compiled to generate movies.
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