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RNeasy (QIAGEN) and ReverTra Ace (Toyobo) were used to prepare cDNA from embryos. The primers used for amplification are shown in Table S2 in File S2. The PCR products were electrophoresed using a DNA-1000 kit on MCE-202 MultiNA (Shmadzu).
Copyright and License information: ©2017 Kimura
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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