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To exclude the temporary effects of treadmill exercise, sacrifice was conducted after 48 hours from the last exercise session. After complete anesthesia (ethyl ether), blood samples (5 ml) from the abdominal vena cava were obtained in syringes. Plasma was collected by centrifugation of heparinized blood at 3000 rpm for 15 min. Soleus muscle were removed and stored at -70°C until analysis.
Copyright and License information: ©2016 The Korean Society for Exercise Nutrition
©2016 Ju Yong Bae et al.; Licensee Journal of Exercise Nutrition and Biochemistry. This is an open accessarticle distributed under the terms of the creative commons attribution license (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the orginal work is properly cited.
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