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Cortices were homogenized in RIPA buffer on ice and an equal volume of 2X SDS sample buffer was added before sonication. Samples were maintained on ice throughout before loading on SDS-PAGE. TMEM106B protein runs as a dimer under this condition (Additional file 1) [7]. Western blots were done as previously described [6]. TMEM106B protein levels were quantified using LiCor Odyssey system and normalized to beta III tubulin.
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