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Tumour cells (5 × 103) were plated in dishes and cultured at 37°C for a week and then stained with crystal violet for 20 min. and further photographed. Alpha Innotech (San Leandro, CA, USA) imaging software was used to quantify the number of cell colony.
Copyright and License information: ©2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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