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Image processing

MS Marijke Scotuzzi
JK Jeroen Kuipers
DW Dasha I. Wensveen
PB Pascal de Boer
KH Kees (C.) W. Hagen
JH Jacob P. Hoogenboom
BG Ben N. G. Giepmans
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In Fiji (http://fiji.sc/), three selected elements were merged as RGB. All image processing has been applied to the whole image in a linear fashion using Fiji and/or Adobe Photoshop. To discriminate 10 nm immunogold and QD655 nanoparticles upon immunolabeling of amylase and insulin, semi-automated annotation using the Fiji macro “golddigger” was performed35.

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