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MF Maria E. Falzone
ZF Zhang Feng
OA Omar E. Alvarenga
YP Yangang Pan
BL ByoungCheol Lee
XC Xiaolu Cheng
EF Eva Fortea
SS Simon Scheuring
AA Alessio Accardi
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All the data analysis were performed using JPK data processing software. The Young’s modulus of the supported lipid bilayers was derived by fitting the force distance curves with the following model54,55,

where F is the Force, E is the Young’s modulus, n is the Poisson’s ratio, d the indentation (vertical tip position), and α is the half cone angle or half face angle of the pyramidal tip shape.

The area stretch modulus (kA) and bending stiffness (kc) of the supported lipid bilayers were calculated based on the measured Young’s modulus (E) and membrane thickness (h) using the following equations (the Poisson’s ratio n is assumed 0.556):

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